Publications - Published papers

Please find below publications of our group. Currently, we list 563 papers. Some of the publications are in collaboration with the group of Sonja Prohaska and are also listed in the publication list for her individual group. Access to published papers (access) is restricted to our local network and chosen collaborators. If you have problems accessing electronic information, please let us know:

©NOTICE: All papers are copyrighted by the authors; If you would like to use all or a portion of any paper, please contact the author.

Downregulation of IGF2 expression in third trimester placental tissues from Zika virus infected women in Brazil

Andréia A. Suzukawaa, Camila Zanlucaa, Natasha A. N. Jorgeb, Lucia de Noronhac, Andrea C. Koishia, Caroline B. V. de Paulac, Patrícia Z. Rebutinic, Seigo Nagashimac, Aruana F. F. Hansel-Frosed, Vinícius S. C. Parreirad, Juliano Bordignona, Margaret R. MacDonalde, Charles M. Ricee, Fabio Passettid and Claudia N. Duarte dos Santosa

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PREPRINT 20-007:
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Status: Published


Journal of Infection

Abstract


Objectives Screening for genes differentially expressed in placental tissues, aiming to identify transcriptional signatures that may be involved in ZIKV congenital pathogenesis. Methods Transcriptome data from placental tissues of pregnant women naturally infected with Zika virus during the third trimester were compared to those from women who tested negative for Zika infection. The findings were validated using both a cell culture model and an immunohistochemistry/morphological analysis of naturally infected placental tissues. Results Transcriptome analysis revealed that Zika virus infection induces downregulation of insulin-like growth factor II (IGF2) gene, an essential factor for fetal development. The Caco-2 cell culture model that constitutively expresses IGF2 was used for the transcriptome validation. Asiatic and African Zika virus strains infection caused downregulated IGF2 gene expression in Caco-2 cells, whereas other flaviviruses, such as dengue serotype 1, West Nile and wild-type yellow fever viruses, had no effect on this gene expression. Immunohistochemical assays on decidual tissues corroborated our transcriptome analysis, showing that IGF2 is reduced in the decidua of Zika virus-infected women. Conclusions Our results draw attention to IGF2 modulation in uterine tissues, and this finding is expected to support future studies on strategies to ameliorate the harmful effects of Zika virus infection during pregnancy.

Keywords


Zika virus, placenta, transcriptome, Insulin-Like Growth Factor II, decidua