Table S21: Summary of double guide box H/ACA snoRNA families. Precise target information such as alignment and single sequence position, ICI scores, number of detected interactions (\#ia) and mean minimum free interaction energies are shown for both hairpins. Experimental verification of target interactions is shown in the column 'verified'. In case the annotated target shows not the highest ICI scores, the best predictions and the verified targets are denoted.

original name	# sequences	hairpin	aln pos	sce pos	conservation	ICI_sno	# interactions	mean mfe	verified	snoStrip name
Nc_HACA_2	82	HP1	25S-3013	25S-1890	Aga,Schiz,11	1.07	42	-28.54	+	HACA_2
		HP1	18S-964	18S-861	Aga,Schiz,11	0.85	50	-27.02	-
		HP2	25S-3541	25S-2351	Aga,9,11	1.01	41	-31.83	+
snR82	9	HP1	25S-2125	25S-1111	-	0.38	3	-45.71	+	HACA_31
		HP2	25S-3541	25S-2351	10	1.09	8	-28.29	+
snR81	3	HP1	U2-62	U2-38	Sacch	1.30	3	-41.19	+	HACA_26
		HP2	25S-2060	25S-1052	Sacch	1.32	3	-35.02	+
snR5	31	HP1	25S-2139	25S-1124	Schiz,10,Neu	1.01	23	-22.97	+	HACA_27
		HP2	25S-2005	25S-1004	Schiz,10,Neu	1.04	22	-30.75	+
snR49	22	HP1	18S-131	18S-121	Scm,10	1.07	18	-25.66	+	HACA_29
		HP1	25S-3048	25S-1925	10	0.71	14	-21.06	-
		HP1	25S-1991	25S-990	Scm	0.56	10	-24.14	+
		HP2	18S-342	18S-302	Scm.10	0.92	17	-29.32	+
snR3	116	HP1	25S-3311	25S-2129	6	0.74	51	-42.79	+	HACA_3
		HP2	25S-3449	25S-2264	6	1.03	70	-31.31	+
		HP2	25S-3315	25S-2133	6	0.90	61	-33.45	+
snR83	4	HP1	18S-1218	18S-1097	Sacch	0.79	3	-30.51	-	HACA_30
		HP1	18S-1566**	18S-1414	Sce,Spa	0.47	2	-26.26	+
		HP1	18S-1567**	18S-1415	Smi	0	1	-27.88	+
		HP2	18S-1419	18S-1290	Sacch	1.32	4	-24.73	+
snR80	100	HP1	18S-852**	18S-758	Puc,10,11-Sor	0.63	42	-31.02	-	HACA_37
		HP1	18S-853**	18S-759	Puc,10,11-Sor	0.33	21	-26.52	+
		HP2	25S-1761	25S-776	10,11	1.12	55	-37.49	+
snR161	9	HP1	25S-2015	25S-1014	Sac	0.70	6	-27.59	-	HACA_39
		HP1	18S-860	18S-766	Yli,Kpa,Sac	0.65	6	-25.67	+
		HP2	25S-4257**	25S-3013	Sac	0.79	6	-29.78	-
		HP2	18S-685**	18S-632	Yli,Kpa,Sac	0.49	4	-25.43	+
		HP2	18S-686**	18S-633	Sac	0.50	4	-26.75	-
AJ632008	4	HP1	25S-3500	25S-2314	Schiz	1.27	3	-26.69	+	HACA_46
		HP1	U2-58	U2-34	Schiz	1.26	4	-23.14	-
		HP2	25S-3394	25S-2211	Schiz	1.10	3	-29.76	+
snR8	100	HP1	18S-775	18S-699	Sacch,Can,11-Hyp	0.43	33	-24.36	-	HACA_6
		HP1	25S-1956	25S-960	Sac	0.39	24	-22.38	+
		HP2	25S-2139	25S-1124	11	0.73	37	-28.49	+
		HP2	25S-1987**	25S-986	Deb,Met,Can	0.17	8	-26.71	+
		HP2	225S-1988**	25S-987	Yli,Sac	0.16	9	-39.50	+

Conservation of target interactions: 1) Fungi 2) Microsporidia 3) Mucoromycotina 4) Chytridiomycota 5) Blastocladiomycota 6) Dikarya 7) Basidiomycota 8) Asomycota 9) Taphrinomycotina 10) Saccharomycotina 11) Pezizomycotina 12) Dothideomycetes 13) Eurotiomycetes 14) Leotiomycetes 15) Sordariomycetes Aga) Agaricomycetes Puc) Pucciniomycotina Schiz) Schizosaccharomycotina Scm) Saitoella complicata Deb) Debaryomycetaceae Yli) Yarrowia lipolytica Met) Metschnikowiaceae Sac) Saccharomycetaceae Kpa) Komagataella pastoris Sacch) Saccharomyces Can) Candida Hyp) Hypocreomycetidae Sor) Sordariomycetidae Neu) Neurospora 3..6) equals 3,4,5,6 6-15) equals 6 without 15